Chain of custody form, 21 West Street, Nov 2001

NYC 9/11 Public Portal Document FEB-28-2002 THU 11 19 AM CLOSINGS 0 ROSE FAX NO. 212 210 6724 P. 37/42

Rose Associates JLC Project # 01-2089 September 18, 2001 page 2 it SarnplinQ Analysisand Procedure

Introduction Air sampling is defined as the process of measuring the filter content of a known volume of air eniralned on a filter membrane over a specified period of time. Each sample is cross-referenced by field designation and a JLC lab internal code to ensure proper, consistent handling through a chain of custody, Sample preparation takes place within an HEPA filter clean bench to avoid any pocclblc contamination.

Methodology Test procedures for these samples conform to the National Institute of Occupational Safety and Health (NIOSH) standards. ;l, Air sampling was conducted at a level approximating the breathing zone of persons anticipatpri to be present in the area during normal operations. Samples collected should represent the area's typical airborne fihr►r concentration. All samploc wore collected on fully exposed 25 millimeter, 0.8 micrurrieter (pm) cellulose ester membrane air sampling cassettes with conductive cowl.

Analytical Procedures Al oeotion of the filter approximating twenty-five percent of the total filter was removed and placed on a ,pre-cleaned microscope slide and then exposed to approximately 250 microliter (pl) of acetone vapor. A single 3.0 - 3.5 pl drop of triacetin was applied and a clean #1.5 cover slip was placed upon the filter. The prepared slide was then heated on a slide heater until clarity allowed analysis to he conducted, A►rlalysis was accomplished utilizing a Phase Contrast Microscope in accordance with NIOSH 7400 method, counting lute "A."

Phase Contrast Microscopy JLC Environmental's laboratory uses an Olympus BH-PC Phase Contrast equipped with a Walton-Beckett Oraticule, Samples were analyzed by firstMicroscope, (PCM) dissolving the filter through a chemical process (described above) and then counting the fibers with PCM at 400x magnificatipn. Phase contrast Increases the light contrast between the object and the thus enhat~cing the microscopist's ability to coo fibers. Normally, 100 microscopic fieldsbackground, or 100 fibers are counted, whichever occurs first,

Analytical Results The "Su.umrnary of Analytical Results" represents the results of the analysis of the samples. The PCM limit of reliable quantification is less than 0.01 fibers/cubic centimeter (f/cc) of air, If final tests do not meet these requirements, the entire work site must be re-cleaned and re -tested,

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