NYC-WTC_000208957

NYC 9/11 Public Portal Document

Jun ID 02 OatOVp Aintek Environmental Corp (212)788-0753 p. 8 •FEB-02-2002 17 = 44 SCltNUi-ic LHBb ----------------

Fl»c» the three ToM grid sample filu r prepara- TEM at a calibrated soecn niaciifuarioii of between i S OOO iioRs on tne ante piece of leas tissue in the JatTs washer. aod 20 OOOX. Tjuioally measure one grid opeaics for each 12.6.2.3 Rice the lid on the JaiTe washer and allow the gj^ eMKUtaed. Mestsun grid openitigs lit both the x and y jyslem to Stand for several hcun. di/ectidAS and calculace the arsa. 12.7 Alternately, pfaus the grids on a tow level Kin dish 14,4 ?Tc-calibrated TEM gridi are also acceptable for this tilled to the 7» nurtt) DMF lafTe washer for $0 mix Add tstt»«hed. enough solutioa at equal parts OMf/aeetone a SU the washer to foe tenea level Remove foe grids after 30 oiiu if IS, TEM Mtthwl • they have cleared, that Is, all filler meteruil has beei feia<i>ed iS.l Microscope seta tigs 80 to 120 kV. 13 000 w ' 1 from [he carbon fttra, as daenttiewd by iia^eetiiw in ihe J 20 OOCX screen magniflcatian for analysis (7-2). TEM. 15.2 Analyze (wo grids for each sample. Analyze one-half 12.8 CvetuUy xmow the grids from the lari ; wssher, of the sample uu on one sample grid preparation and the . JlJowing foe grids (a dry before phicine them i i r dean rcjnainlug half on a second sample grid prtpamiOa. roarlced grid pox. 15.3 DtttrminuiaA of Spedntfn 13. TEW Specitnen Preparadoa of Polycarbonate (PO 15.3.1 Caxclully lend ±b TEM grid, carton side facing up tin the T£M column) with the grid ban oriented paraUeU filter perpendicular to Che length of the ipeacneo holder. Use a 13,1 cover the surSuc of a clean urioroscope ilide with band leu or loupe, ■( neetuaiy. This proeadure wjU tine up [WO strips of dnubio'sided adhesive tape. the grid with the X and y tnasiarien dinroiou of the 13.2 Cut a strip of alter paper slightly nanowe thu foe microscope. Insert this speriroen holder Into the microseope- width of foe slide. Poatioa the filter paper strip on iK ctnur 15.3.2 Scat) the emiw grid at low magniScacion (x50X co of foe length of the slide. 1 OOOX) co determine its suitability for high magailictrion 13.3 Using a elsaa, curved scalpel bhde, cut a st ip of foe aaalyaia as specified in I i.3.3, PC ailer approxitBai^y 25 by 6 mm. Use a rackia atodoa 15.3.3 Grids are acceptable for ualyiis if the follawiag or the scalpel blade lo avoid tetuiAg the filter. Rac; the PC soodJtiQM are met: strip pxrtids side up on the slide perpendlcutw lo foe long 15.3.TI The (faction of grid openings covareJ by the Olis of the Slide The ends of foe PC strip roust co itact foe replica section is at least 30 %. double sided adhesive tape- slide can hold se 'eral PC IS J, 3.2 ReJativc to, that secrioo of the grid covered by the scrips. Wiri) 3 stass Rurker, late) ncri ?c strip rich Ite carbon replica, the fraciiaa of intact grid openings is greater individual sirople number. than SO %, 13.4 Carton coat the PC filter strips ax disci sud io JJJJ.3 The fractitinaJ area of uadisscived filui is les 13.1.2. PC Slurs do act require etching. thaa 10%. Nore 3: Cred**—Oo b« overheat foe Slur jecdooi wh! e tartea 15J J.4 The firactioo ofgrid opeaiags with ovoAifipitig or cauing. folded eeplie* film is less than 50 %. 13.5 pTcpue a lafe washer 8S desentod in 12.5 but SU IS,3.3J At hast 20 grid openings, that have w omlap- ths wether with cWoroform or l-aethyl-2<pyn-olidoi e u foe plag or folded replica, are len ±4B 5 35 covered with holm and have las fobs S % opaque area due to incomplete (Ute: level of foe screen. 13-6 UsiBg a dean curved scalpel bladA «ei»i three, diflolution. 3-mtt square Slur pieces iiom each PC scrip. Plane tto iHtr IS.4 Dttermi/i^ton Grid Opening SuiiabiHiy: squares carton ride up on the staty site of» TEM 9 4 Piek 13.4.1 If foe grid meets acceptabce criteria. Choofc a gric up the grid and fitter seetion together and place then so foe opening for analysis foam vtodos areas of tte zrid so that foe eatfoe grid is represcacsd. Determine foe zuiiabillty of eacn leas dxxue in foe Jaffe washer. 13.7 Flace foe lid 00 foe Jafte washer and rest foe Wtefo individual grid opening prior to the analysis. pUee for « letn * h. Best results are obtained witi longer 15.421 Ito ibdiYdual grid opening roust have les than widdag tiroes, up to 12 h. 5 % holo over its area. 13.4 Cam&dly remove the eridi frem the Ja^e inster. 15.4.3 Grid opcaiogj roust be less than 25 % covered with illowing the grids to dry before placing them io s dm. pariKUlaie matter. 15.4.1 Grid opening!; must te uniformly loaded. marltcri arid box. 15.5 Observe and reuonj foe arietaaiion of foe grid at SO 14, Grid Openiag Measvemcao to 150X, on « grid mac n»<ri then along with foe loauoc 14,1 TEM grids mua have i known ©id opcawfe ana. of foe grid openings that an cMnwed for foe aaalysis. If indexed grids are used, a pid aup is not required, but foe Dctenaiae this a«a a» follows: ideaefying coordinaies of foe grid squre lawt be recorded. ) 14.2 Mmwe at least 20 grid openings in each « !0 I fMdom 75 w lOO SOB pt06.aiesh) tapper grids foe x lotel of 16. Bccardbe Daa Saia 1 400 grid openings (br every 1000 grids used, by pUe eg foe 20 grids OU a glass slide sod arominj^ them uat er foe 16.1 Rocorf OR the count sheet any coaciauous gioupuig optical rakroscopc. Ux a caiitwaied gtaricule to mea re the of parocSea is which an ateasut fiber is detected. daasiV average l«grii andwidfopffoe 20openingsfttjajeaefc offoe asbedo* Aueuuee aa fibm, bundto. dusien. at tsaniccs as individual gride. From the aasumulaicd data, calculfc the deftnedie 5.2- average grid opening wet of foe 400 openings. 162 Use the criteria for text, buadle, cluster, and matrix 14J Grid urea memacmeots can siM to made H [te idanrifieation, as described in the 6’S£?zl Aib^siOf-Cvaauting

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NYC-WTC_000208963