NYC-WTC_000208957

NYC 9/11 Public Portal Document

Jun 10 02 02s0Gp Rirtek Environmental Corp f?13)7Kn flEB-g?-201311' I ir 4 J /dH--0759 P-7 05755 12-1,1 Rcn»ve * section (a plug) froni any quadiaht of mil be tucto Cartes rods (7.40) ujsed far evaponian shall be rhe ample Md blank fites, SeclioM css be removed trom shitpcBcd with a earbon rod xharpeaer to a neck of about 4 the files uxJbj a 7 mm cork bora (7J2). The cori: sorer nun ia lei^ and 1 to 01 in dUmettf. The rods «t intallad iDua be wiped after each tinie a section a rtaaoye^ to the evaporator is suKto a ruanae that toe potou art liJ,2 PbtB the filter aeadoe (partidt ride up) od a Uten apncraunutiely lOO to 120 mm dom the snrftce of toe mieresoope iCde. Affix toe Sha jetssce to toe tode wtth a aicroscope slide held to toe roaung device, gunned page nriofoKweat (7.43), ee oton JuiSabie tB^atw. 1X42 Place the glai. slide hoWinj toe fllaw oa toe Uto the dito with 8 glass scrihise »Ol dr perm^ect rotabon device, and tivacoate toe evaporator chamber to a marker (7.10). i vacuum of at least 13 WFa Perform toe evaporation in very 171.3 Prepare a fusing dish Crore a glass pern dish n.37) short bursa, separated by 3 to 4 s to allow Use elKiroaes to and a metal sdbcb bridge (7 Jg) with a pod of five to six cool. Ab afterpaK netood of evaporadoo is by using a slow ishlcss paper Bilm (7,42) and place io the bcuom o( the eontinuoQS applied curteat. An experienced analyst ea.c peiTi dish (4). FSacs the sown bndge on top of the pad and judge the toidenos of toe carbon film to be applied. Conduct samndc the Sltei pads with icaiooc. Flace the dide oa u p of tests OB unused Citcjr first. If toe carton Ehn is too toil, the bridge ia the peai dish aad cover the dish. Wait large particles will to lost Grom toe T£M spedaeB, and toerc apprruiraately S tain for toe sample filter to fuse sod clbar. will be few eeiBpleie and undaataged grid Opening! os toe 12.2 Dbruthy^ormairudt-jteefif MetftOil: IXII Place a drop of clearing soiuaoc that cottas^ s of I2.4.2.I If toe coating is too thick, it will lead to a TEM 35 % dimetoyLtoroasiide (DMF), 15 % glacial acetin : dd. itsaee tool is lacidos a wntrast, nd toe ability to obtain and 50 R Type II water (v/v) an a deas microjeope tode- electron dif&arsioc. panems will to comptomhecl- The Gauge toe amcuni used so that toe cteariog solution {just carton film shall to as thin u possible and still remain intsdl aturaies toe fiber seetion. I on most of toe grid openings of toe TEM spectrnen, 12.2-2 Carenilly lay the filter segrocm. sample tuiffijct 12.3 A'rportwfroo ffihr JtiSf iVasher—The precise design upward, OB cop of the solution. Bring toe filler and soliton of toe laffe washer is not considered itnpcrani, so any one of cogetoerat an angle of about ?0‘ to Mp exclude air bubbles. toe pubiisbed toagns may to used (7,8). Ooe such washer Remove any eicess clearing sotudon. Fiae* the slide it an oaostsis of a simple stainless sted bridge contained In a gltis □ven or on a hoi plaie. io a ftirae hood, a 65 to 7O'C fo 10 peuitiish- min. 12.3.1 Race several pieces of lens tissue (7,41) on the 1X3 Plasma etching of the collapsed filter is tEquired staioiess sael brkSge, The pieces of Isos U«ue shall to large 13.3.1 The fflierwoope slide to which the OolUpsad iUtcr enonsit w completely drape ovar the bridge and into toe faeces are attached is placed io a plasma asher (7. !7). sohcBb In 1 fume hood, GD too petii dish with isceiose (or Secnose ptastBs ssb^it vary gmily io Uteir ptefomta la, DMF) anol toe height of the solvent is brought up to eoniaa both From unit lO unit and between differer; positions in the the undenide of toe metal bridge as ilJusuaied in Fig. 2. isher chamber. i< is tJiflioiH to specify the exact coidiL pjis 12.6 the Spfcimeni iiao iheJ^ffe Washer that must be used. lasufScieai will leauh in a fa ur« 13.6,1 tnaee the TEM grids (7J$) Shiny side up 00 a place to expose embedded Sben, and too much etching may ra mil of Ions tissue or Gher paper so that individual grists car to in the Ina of panicles from the filter xuriacc. To deurn iae easily picked up with tweezws. the Opel mure line for ashing, place aa unused 25 mm 12.62 Prepare three grids from each sample. diamR« MCE Slw io thi cenwr of a gtm nlcroiwpa *1 de. 12.6.2.1 Uries acuned scalpel blade (7 20X excise, at least PnsitioD the slide approntnatcly in the center of the at her two square (j mni by 3 mtn) pieces of toe cartjon-ooaicd chamber. Oose the dumber and evaesutt to a prssiun of MCE filter ffOB toe glass slide, appraxiaaatclj 40 Pa, while admitting axygea to the chao^ 12,67.2 Place the square filter piece carbon-side up on at a nse of 3 to 20 em’/mia. Adjust toe tuninj of toe syr ent top cfaTEM specitaen. grid- to that toe imcnaty of toe plajt^ is naaiiaized. Detent ine I2.fi.X3 Place the whole assemelv (filier/Erid) on toe the time requireti for QOmpICU OiddatiOD of toe fUar. Ad. '431 saturareti less tissue is toe laffe washer. the system parameters to achieve complete oxidation of toe lifter in a period of appronatately 15 mia. For etchins of collapsed filters, use these operating panneton for a per pc «ili>onop« of $ min. For addisfooal uaformaoon on calibration, see__ »« Gau skvl san -a.ciotiw tt*iSMBtSUttn*i» OSEW Aebtstes-Co/tUuning hfatendj in SeJiocU (4) Or Itou »>« « 'S "'■I hfMV IIS n»tw Progrem //aadbwk /or Aithcme AshiiVK ARoJyw (<) daeuraesis. 12.3.2 Place the glass slide coatainins toe collapsed fin into toe l«w-ieniperaurre plisrna asher, and etch the filto: 12.4 Cartes eaetitig of toe coUapsed end etahed £fter iis required. I I 12.4,1 Carbon coaiinj must be peT-'ormed with s iu, I '^uum omting unit (7.4), eapuble of las than ID"* torr 113 Um - ( MFi) presture. Units that are based on evapnraiioo Taru* Kdbon ntaoeats in a vacuum geoormed o^y by la Ai) row I pump have not been evaluated for thw appiicaiaon and shiall RS. : EeampH «X Dwsn at Sealant Wa<4>»r (J«»U Waaha; J !

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