VOC sampling locations table for Ground Zero area, Jan 2002

NYC 9/11 Public Portal Document

Protein cza Saline Albumin 1=1 Saline a E=H3MSH-1OO 50-1 E=3MSH-1OO 300-1 Bram ROFA -10 ROFA -10 ■■a ROFA -100 a ^■B ROFA - 100 250- I ; M 200- 1^^ WTCX - 10 ESSS3 WTCX-31.6 asc : WTCX - 10 Esssa WTCX-31.6 Essa WTCX - 100 A Esa WTCX - 100 3 a 150- .2 25- s ■

50-

0 0

LDH NAG Saline 3 Saline 120-1 10 laMHi MSH-100 a 3 MSH -100 a Essssi rofA -10 ROFA-10 ■■■ ROFA -100 8 iHH ROFA -100 ESza WTCX - 10 g WTCX -10 WTCX-31.6 6 3 WTCX-31.6 Esa WTCX -100 ESffia WTCX -100

I.- X S 4 2

0 0 Figure 7. Experiment A Values for total protei n, lactate dehydrogenase (LDH), albumin, and N-acetyl-b-D-glucosaminidase (NAG) were measured in bronchoalveolar lavage fluid supernatants recovered from mice one day after exposure by intratracheal instillation to PM samples in saline or saline vehicle alone. Values shown are means and SEM (n=12 per group). ^P< 0.001 vs. Saline group.

other reason could be deduced for tiie difference. saline control group and both the MSH-100 group and the Consequently, we performed a third sub-experiment to WTCX-100 group (Table 8, Figure 6; P < 0.05). examine these endpoints and increase the n umber of mice Neutrophils comprised about 7% of total BAL cells in the per group (sub-experiment A5). The average total cell WTCX-100 group, but only about 1% or less in the number recovered from saline control mice in A5 was 18 WTCX-31.6 and WTCX-10 groups. X IO'* (97% AMs) - about in the middle between Al and Levels of proteins and enzymes were measured in the A2. All data shown is combined from the 3 sub­ BAL supernatant to assess lung damage. Both total protein experiments. Due to the high variance of data in the and albumin are increased after damage to the alveolar ROFA-100 group, we judged that it was necessary to epithelial barrier (Henderson et al., 1985). Lactate compare ROFA-100 data alone vs. saline control data. dehydrogenase (LDH) is a cytoplasmic enzyme which is Other comparisons were made between the saline control released by dead or dying cells, while N-acetyl-P-D- group and the other groups after excluding ROFA-100 glucosaminidase (NAG) is indicative oflysosomal enzyme data. Significant increases in neutrophils, eosinophils, and release (Henderson et al., 1985). All of these parameters lymphocytes were found in ROFA-100 mice compared were significantly increased in the ROFA-100 group in with saline control mice (Table 8). Neutrophils comprised comparison to saline control mice (Table 8). Total protein 31% of total BAL cells in ROFA-100 mice (Figure 6). and albumin were both increased about 80% compared to After excluding the ROFA-100 data, significant saline, while LDH was increased 3-fold and NAG almost differences in neutrophil numbers were found between the 4-fold (Figure 7). No significant changes in BAL proteins

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