NYC-WTC_000199935

NYC 9/11 Public Portal Document

LiphaTech 90225 Page 10 of

The relative humidity was stabilized at 20-55%. Both the temperature and humidity were monitored by a Serdex Temperature and Relative Humidity Recorder. A 12-hour light/dark cycle starting at 6:00 AM using artificial lighting not exceeding 200ft candles at cage location was maintained. The foot candles were measured by using a General Electric Type 214 Light Meter. Animals were supplied with tap water, The water bottles were 802. French Square Glass Bottles from W.B. Bottle Company, 836 E. Bay Street, Milwaukee, WI 53207. The Rubber Stoppers (#7) were from Fisher Scientific, 1600 W. Glenlake, Itasca, IL 60143. Stainless Steel Sipper tubes from Unifab Corporation, were inserted into each rubber stopper and placed in each water bottle. The water bottles were checked daily. The laboratory-and animal room were maintained as limited-access facilities by authorized personnel involved in the testing. 6.3 Justification for the Selection of Animal Species. Mice were used in this study, because the purpose was to test the palatability and effectiveness of a-rodenticide. Also, the.y are preferred by the. U.S. Environmental Protection Agency. 7.0 TEST PROCEDURES. 7.1 Quarantine and Identification of Animals. Upon receipt, animals were placed in quarantine for 7 days, under the same conditions as for the actual test. While in quarantine the animals were fed Purina Rodent Lab Chow, purchased from Waldschmidt and Sons, Menomonee Falls, WI 53051. 7.2 Selection of Animals. Animals selected for the test had not been subjected to any previous experimental procedures, trauma, nor disease. Animals selected for the study were chosen from a larger pool of animals. 7.3 Procedures Upon Selection of the Animals. Selected animals were weighed to the nearest 1.0 gram; the data was recorded on each data record sheet. 7.4 Selection of the Dose. In order to provide in excess of the daily food requirement of'2 grams per animal per day minimum, a dosage of 120 grams of test substance per cage per day was selected. 7.5 Control Groups. An untreated control group consisting of 10 males and 10 females was used concurrently with the test group. 7.6 Preparation of the Test Animals.

NYC-WTC_000199938